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F asciola hepatica tegumental coat antigen suppresses MAPK signalling in dendritic cells and up‐regulates the expression of SOCS 3
Author(s) -
Vukman K. V.,
Adams P. N.,
O'Neill S. M.
Publication year - 2013
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12033
Subject(s) - biology , signalling , immunology , microbiology and biotechnology
Summary F asciola hepatica tegumental coat antigen (FhTeg) suppresses dendritic cell maturation and function by inhibiting IL ‐6, tumour necrosis factor ( TNF )‐α, IL ‐10 and IL ‐12 production and CD 80, CD 86 and CD 40 cell surface marker expression in TLR 4‐stimulated dendritic cells. Fasciola hepatica also impairs dendritic cell function by inhibiting its phagocytic capacity and its ability to prime T cells. We have shown previously that activation of mast cells with bacterial ligands is also inhibited by FhTeg. Fasciola hepatica suppresses LPS ‐induced NF ‐κB and MAPK pathway ( ERK ) activation in these cells. Previously, we demonstrated that FhTeg induces expression of suppressor of cytokine signalling ( SOCS )3, a negative regulator of the TLR pathway in mast cells. In this study, we show the same inhibitory effect of FhTeg on the activation of the other members of the MAPK s pathway ( ERK , p38, JNK ) in dendritic cells and demonstrate an enhanced expression of SOCS 3, but not SOCS 1, SOCS 5 or PIAS 3 in this process. These studies enhance our understanding of the immunomodulatory effect of helminth molecules on the TLR pathway.