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DEAD‐box proteins, like Leishmania eIF4A, modulate interleukin (IL)‐12, IL‐10 and tumour necrosis factor‐alpha production by human monocytes
Author(s) -
Barhoumi M.,
MeddebGarnaoui A.,
Tanner N. K.,
Banroques J.,
Kaabi B.,
Guizani I.
Publication year - 2013
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12026
Subject(s) - biology , dead box , tumor necrosis factor alpha , immune system , immunology , recombinant dna , cytokine , monocyte , virology , rna , helicase , genetics , gene
Summary Previously we showed that His‐tagged, recombinant, Leishmania infantum eukaryotic initiation factor (Le IF ) was both an RNA ‐dependent ATP ase and an ATP ‐dependent RNA helicase in vitro , as described for other members of the DEAD ‐box helicase family. In addition, we showed that Le IF induces the production of IL ‐12, IL ‐10, and TNF ‐α by human monocytes. This study aims to characterize the cytokine‐inducing activity in human monocytes of several proteins belonging to the DEAD ‐box family from mammals and yeast. All tested proteins contained the 11 conserved motifs (Q, I, Ia, GG Ib, II, III, IV, QxxR, V and VI) characteristic of DEAD ‐box proteins, but they have different biological functions and different percentages of identities with Le IF . We show that these mammalian or yeast recombinant proteins also are able to induce IL ‐12, IL ‐10 and TNF ‐α secretion by monocytes of healthy human subjects. This cytokine ‐ inducing activity is proteinase K sensitive and polymyxin B resistant. Our results show that the induction of cytokines in human monocytes is not unique to the protein Le IF of Leishmania , and it suggests that the activity of certain DEAD ‐box proteins can be exploited as adjuvant and/or to direct immune responses towards a Th1 profile in vaccination or immunotherapy protocols.