Premium
A proteomic approach to identify proteins from Trichuris trichiura extract with immunomodulatory effects
Author(s) -
Santos L. N.,
Gallo M. B. C.,
Silva E. S.,
Figueiredo C. A. V.,
Cooper P. J.,
Barreto M. L.,
Loureiro S.,
PontesdeCarvalho L. C.,
AlcantaraNeves N. M.
Publication year - 2013
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12025
Subject(s) - trichuris trichiura , biology , peripheral blood mononuclear cell , immunology , heat shock protein , trichinella spiralis , helminths , biochemistry , ascaris lumbricoides , in vitro , gene
Summary Infections with Trichuris trichiura and other trichurid nematodes have been reported to display protective effects against atopy, allergic and autoimmune diseases. The aims of the present study were to investigate the immunomodulatory properties of T. trichiura adult worm extract (Tt E ) and its fractions (Tt EF s) on the production of cytokines by peripheral blood mononuclear cells and to identify their proteinaceous components. Fourteen Tt EF s were obtained by ion exchange chromatography and tested for effects on cytokine production by peripheral blood mononuclear cells. The molecular constituents of the six most active fractions were evaluated using nano‐ LC /mass spectrometry. The homology between T. trichiura and the related nematode Trichinella spiralis was used to identify 12 proteins in Tt EF s. Among those identified, fructose biphosphate aldolase, a homologue of macrophage migration inhibitory factor and heat‐shock protein 70 may contribute to the immunomodulatory effects of Tt EF s. The identification of such proteins could lead to the development of novel drugs for the therapy of allergic and other inflammatory diseases.