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Differential immune response in mice immunized with the A, R or C domain from Tc SP protein of Trypanosoma cruzi or with the coding DNA s
Author(s) -
SalgadoJiménez B.,
ArceFonseca M.,
BaylónPacheco L.,
TalamásRohana P.,
RosalesEncina J. L.
Publication year - 2013
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12017
Subject(s) - recombinant dna , biology , parasitemia , dna vaccination , virology , immune system , trypanosoma cruzi , microbiology and biotechnology , antibody , immunization , plasmid , dna , immunology , gene , plasmodium falciparum , genetics , parasite hosting , malaria , world wide web , computer science
Summary In a murine model of experimental Trypanosoma cruzi (H8 strain) infection, we investigated the induction of protective immunity against the domains [amino (A), repeats (R) and carboxyl (C)] of the surface protein (SP), a member of the trans ‐sialidase (TS) superfamily. Recombinant proteins and plasmid DNA coding for the respective proteins were used to immunize BALB/c mice, and the humoral response and cytokine levels were analysed. Immunization with the recombinant proteins induced higher levels of anti‐TcSP antibodies than immunization with the corresponding DNAs, and analysis of serum cytokines showed that immunization with both recombinant proteins and naked DNA resulted in a Th1–Th2 mixed T‐cell response. Mice immunized with either recombinant proteins or plasmid DNA were infected with blood trypomastigotes. The recombinant protein‐immunized mice showed a variable reduction in peak parasitemia, and most died by day 60. Only the p BKT c SPR ‐immunized mice exhibited a significant reduction in peak parasitemia and survived the lethal challenge. DNA‐based immunization with DNA coding for the repeats domain of TcSP is a good candidate for the development of a vaccine against experimental T. cruzi infection.