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Immunoregulatory profile of monocytes from cutaneous leishmaniasis patients and association with lesion size
Author(s) -
Vieira É. L. M.,
Keesen T. S. L.,
Machado P. R.,
Guimarães L. H.,
Carvalho E. M.,
Dutra W. O.,
Gollob K. J.
Publication year - 2013
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12012
Subject(s) - cutaneous leishmaniasis , immunology , biology , association (psychology) , leishmaniasis , lesion , monocyte , dermatology , pathology , medicine , philosophy , epistemology
Summary Leishmaniasis is an important tropical disease composed of several clinical forms that adversely affect millions of people globally. Critical cells involved in the host– L eishmania interaction are monocytes and macrophages, which act to protect against infections due to their ability to both control intracellular infections and regulate the subsequent adaptive immune response. Both soluble factors and cell surface receptors are keys in directing the immune response following interaction with pathogens such as L eishmania . Toll‐like receptors ( TLR s) have an essential role in immune responses against infections, but little is known about their role in human infection with L eishmania braziliensis . In this work, we evaluated peripheral blood CD 14 + monocytes for the expression of immunoregulatory cytokines, co‐stimulatory molecules and TLR 9 from cutaneous leishmaniasis patients infected with L . braziliensis and noninfected individuals. Our results showed that patients present decreased expression of co‐stimulatory molecules such as CD 80 and CD 86 following culture with media alone or after stimulus with soluble L eishmania antigen. Interestingly, TLR 9 expression was higher after culture with soluble L eishmania antigen ( SLA ), suggesting a role of this molecule in immunoregulation of active disease. Lastly, higher frequencies of TLR 9 + monocytes were correlated with greater lesion size. These findings demonstrate a peripheral monocytes profile compatible with important immunoregulatory potential.