Premium
Antigenic differences between the EG 95‐related proteins from E chinococcus granulosus G 1 and G 6 genotypes : implications for vaccination
Author(s) -
Alvarez Rojas C. A.,
Gauci C. G.,
Lightowlers M. W.
Publication year - 2013
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12009
Subject(s) - echinococcus granulosus , biology , genotype , epitope , vaccination , recombinant dna , echinococcosis , immunology , gene , virology , antigen , antibody , genetics , zoology
Summary Cystic echinococcosis caused by E chinococcus granulosus remains an important and neglected issue in public health. The study of the likely efficacy of the currently available EG 95 vaccine against other genotypes of the parasite is important to improve the vaccine as a potential tool to be used in control programmes. The recombinant vaccine EG 95‐1 G 1 was developed based on the G 1 genotype of E . granulosus . Characterization of the eg95 gene family in the G 6 genotype by genomic DNA cloning previously produced the first unequivocal information about the composition of the gene family in a different genotype. The information was used in this study to predict and express two EG 95‐related proteins from the G 6 genotype as recombinants, for assessment of their capacity to bind antibodies raised in sheep vaccinated with the EG 95‐1 G 1 vaccine. The proteins ( EG 95‐1 G 6 and EG 95‐5 G 6) from the G 6 genotype of E . granulosus were unable to bind all the antibodies raised by sheep vaccinated with EG 95‐1 G 1. Differences in the amino acid sequence of EG 95‐related proteins from G 6 and likely the differences in the encoded F n III domain may be responsible for changes in the conformation of these epitopes.