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The antiapoptotic activity of H eligmosomoides polygyrus antigen fractions
Author(s) -
Doligalska M.,
Brodaczewska K.,
Donskow Łysoniewska K.
Publication year - 2012
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12006
Subject(s) - biology , antigen , immunology
Summary Our study identified H eligmosomoides polygyrus antigen factors with potential activity for regulation of T ‐cell proliferation and surviving of CD 4 + CD 25 − , CD 4 + CD 25 hi and CD 3 + CD 8 + cell populations. The antiapoptotic activity of antigenic fractions separated by HPLC was evaluated in vitro after exposure of cells to DEX and r TNF ‐α. Different populations of cells responded to antigen fractions in distinct pattern; the most sensitive population of cells to H . polygyrus products were CD4 + CD25 hi after exposure to DEX and CD 3 + CD 8 + T cells after exposure to r TNF ‐α. H . polygyrus antigens may influence survival of CD 8 + T cells by regulation of c‐ FLIP rather than Bcl‐2, which affects survival of CD 4 + CD 25 hi Treg cells and CD 4 + T cells. Activation of NF ‐κB subunits, for example, p50 and p65 was essential for resistance of cells to apoptosis, and antigenic fractions F 9 and F 17 exerted different effect to F 13. The most active fraction in inhibition of apoptosis was F 9, which includes H sp‐60, calumenin, ferritin, galectin and thrombospondin. This study may provide new clues for recognition of factors that regulate the immune response during infection and which engage the TNF ‐α receptor‐mediated and the mitochondria‐mediated death pathway.