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Apoptotic mechanisms are involved in the death of S trongyloides venezuelensis after triggering of nitric oxide
Author(s) -
Ruano A. L.,
LópezAbán J.,
Gajate C.,
Mollinedo F.,
Melo A. L.,
Muro A.
Publication year - 2012
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12004
Subject(s) - biology , nitric oxide , immunology , apoptosis , pathogenesis , antigen , strongyloides , nitric oxide synthase , strongyloidiasis , microbiology and biotechnology , helminths , endocrinology , biochemistry
Summary Despite progress in understanding the role of nitric oxide ( NO ) in the pathogenesis of helminth infections, the role in strongyloidosis is unknown. Firstly, we studied the production of NO in mice infected with S trongyloides venezuelensis as well as in macrophage cultures stimulated with parasite antigens. Somatic larvae 3 ( L 3) and excretory–secretory female antigens stimulate specific NO production measured by G riess reaction and expression of inducible NO synthase by RT‐PCR and quantitative PCR . Moreover, mice infected with S . venezuelensis produce NO in migration stages. Secondly, we analysed the effect of NO production on L 3 and females of S . venezuelensis using NO donors such as diethylenetriamine and 3,3‐bis(aminoethyl)‐1‐hydroxy‐2‐oxo‐1‐triazene. Parasites died after NO donor treatment in a dose‐dependent manner. Finally, apoptotic mechanisms are involved in the death of S . venezuelensis larvae.