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H eligmosmoides polygyrus fourth stages induce protection against DSS ‐induced colitis and change opioid expression in the intestine
Author(s) -
DONSKOWŁYSONIEWSKA K.,
MAJEWSKI P.,
BRODACZEWSKA K.,
JÓŹWICKA K.,
DOLIGALSKA M.
Publication year - 2012
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12003
Subject(s) - colitis , immunology , biology , opioid , receptor , genetics
Summary Primary exposure of mice to the nematode Heligmosomoides polygyrus infection reduces inflammation in an experimental model of colitis. The aim of the present investigation was to evaluate whether the reduced inflammation provoked by H. polygyrus L4 larvae in BALB /c mice treated with dextran sulphate sodium is associated with changed expression of opioids in the small intestine and colon. Colitis was induced by 5% Dextran sulphate sodium ( DSS ) oral administration for 3 days before oral infection with 200 infective larvae (L3) H. polygyrus until the end of the experiment, 6 days post‐infection. Clinical disease symptoms were monitored daily. The expressions of proopiomelanocortin POMC 1, MOR 1 (Oprm1) – opioid receptor and β‐endorphin were determined by RT ‐ PCR , Western blot and immunoassay, respectively, in the colon and small intestine of mice. RT ‐ PCR analysis of colon tissues showed up‐regulation of the expression of POMC and MOR 1 opioid‐dependent genes in mice with DSS ‐induced colitis. H. polygyrus L4 larvae inhibited DSS ‐induced colitis symptoms that were correlated with increased IL ‐1β, TNF ‐α, IL ‐6, myeloperoxidase ( MPO ) concentration, macrophages infiltration and MOR 1, POMC and β‐endorphin increased expression in the small intestine and inhibition of those in the colon.