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Impairment of the autophagy system in repetitively UVA‐irradiated fibroblasts
Author(s) -
Endo Karin,
Katsuyama Yushi,
Taira Norihisa,
Yoshioka Masato,
Okano Yuri,
Masaki Hitoshi
Publication year - 2020
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1111/phpp.12516
Subject(s) - autophagy , skin aging , microbiology and biotechnology , intracellular , human skin , chemistry , cancer research , biology , medicine , biochemistry , apoptosis , dermatology , genetics
Abstract Background Autophagy is known as an intracellular cleanup system necessary to maintain homeostasis of the skin. Many studies have pointed out the relationship between aging and the inactivation of autophagy function, which suggests that the inactivation of autophagy occurs in aged skin. However, the aging process of the skin is complicated compared with other organs, because the skin is localized at the border between the inside of the body and the environment. Thus, skin aging is strongly affected by environmental factors, and it is well recognized that ultraviolet (UV) radiation is an important environmental factor that promotes skin aging. Therefore, characterizing the autophagic phenotypes induced by environmental factors is important to understand the process of skin aging. Methods In order to demonstrate the status of autophagy during environment‐induced aging of the skin, we investigated the autophagy profiles of normal human dermal fibroblasts (NHDFs) treated with repetitive UVA irradiation as model fibroblasts in photoaged skin. Results Repetitively UVA‐irradiated NHDFs showed increased numbers of autophagosomes, which coincided with the accumulation of p62 and increased levels of LAMP‐1 and lysosomes. The behavior of repetitively UVA‐irradiated NHDFs on autophagy was similar to that of NHDFs treated with hydroxychloroquine (HCQ), which is an inhibitor of lysosomal proteinase. Conclusion In summary, these results demonstrate that repetitively UVA‐irradiated fibroblasts have reduced autophagy function due to the dysfunction of lysosomes.