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Protective effects of Polypodium leucotomos extract against UVB ‐induced damage in a model of reconstructed human epidermis
Author(s) -
Torricelli Paola,
Fini Milena,
Fanti Pier Alessandro,
Dika Emi,
Milani Massimo
Publication year - 2017
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1111/phpp.12297
Subject(s) - epidermis (zoology) , downregulation and upregulation , sunburn , immune system , monoclonal antibody , antioxidant , chemistry , cell growth , microbiology and biotechnology , antibody , immunology , biology , medicine , biochemistry , dermatology , anatomy , gene
Summary Background Polypodium leucotomos ( PL ) exerts potent antioxidant, photo‐protective, and immune‐modulatory activities. A reconstructed human epidermis ( RHE ) (Episkin) is a suitable model for the evaluation of acute UV ‐induced cell damage. No data regarding the photo‐protective action of PL in this model are available. Purpose We evaluated the effects of PL on the prevention of UVB ‐induced cell damage assessing sunburn cells, CPD formation, p53, Ki‐67, p21 expression, and epidermal growth factor ( EGF ) production. Materials & Methods RHE was incubated in standard conditions. PL was topically applied at the concentration of 2 mg/cm 2 , immediately before UVB exposition. UVB exposition (300 mJ/cm 2 ) was performed using a dedicated UVB lamp. Irradiated samples without PL and non‐irradiated samples were used as positive and negative controls. Expression of p53, p21, and Ki‐67 was evaluated with immune‐histochemical methods. CPD were measured using a monoclonal antibody. Results PL significantly reduced sunburned cells (−80%) in comparison with positive control. PL significantly prevented the increase in EGF production at tested times. PL significantly reduced the p53 (−80%), p21 (−84%), and Ki‐67 (−48%) positive cells. Finally, PL prevented the formation of CPD (0% vs. 20% positive cells). Conclusion In this model, PL has shown to prevent UVB cell damage, the upregulation of proliferating proteins, and fully blocking the formation of CPD .