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Inhibitory effect of 660‐nm LED on melanin synthesis in in vitro and in vivo
Author(s) -
Oh Chang Taek,
Kwon TaeRin,
Choi Eun Ja,
Kim Soon Re,
Seok Joon,
Mun Seog Kyun,
Yoo Kwang Ho,
Choi Yeon Shik,
Choi Sun Young,
Kim Beom Joon
Publication year - 2017
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1111/phpp.12276
Subject(s) - microphthalmia associated transcription factor , tyrosinase , melanin , in vivo , depigmentation , downregulation and upregulation , hairless , chemistry , hyperpigmentation , in vitro , mapk/erk pathway , melanocyte , skin whitening , cancer research , melanoma , dermatology , biochemistry , biology , pharmacology , medicine , signal transduction , enzyme , microbiology and biotechnology , gene , active ingredient
Summary Background Skin hyperpigmentary disorders including postinflammatory hyperpigmentation, melasma, solar lentigines, and conditions like freckles are common. The light‐emitting diodes (LEDs) are the latest category of nonthermal and noninvasive phototherapy to be considered in skin pigmentation disorder treatment. Purpose The purpose of this study was to investigate the effects of 660‐nm LED on inhibition of melanogenesis. We investigated whether a 660‐nm LED affected melanin synthesis in in vitro and in vivo models, and we explored the mechanisms involved. Methods The inhibitory effect of 660‐nm LED on melanin synthesis was evaluated in B16F10 cells and HRM‐2 melanin‐possessing hairless mice were used to evaluate the antimelanogenic effects of 660‐nm LED. Results Interestingly, 660‐nm LED inhibited alpha‐melanocyte‐stimulating hormone‐induced tyrosinase activity in B16F10 cells. We also found that 660‐nm LED decreased MITF and tyrosinase expression and induced the activation of ERK. These findings suggest that the depigmenting effects of 660‐nm LED result from downregulation of MITF and tyrosinase expression due to increased ERK activity. The 660‐nm LED reduced UVB‐induced melanogenesis in the skin of HRM‐2 via downregulation of tyrosinase and MITF. Conclusion These findings suggest 660‐nm LED is a potentially depigmentation strategy.