Premium
Theoretical and Experimental Analysis of Protoporphyrin IX Photodegradation Using Multi‐Wavelength Light Sources
Author(s) -
Sánchez Víctor,
Garcia Marlon Rodrigues,
Requena Michelle Barreto,
Romano Renan Ar,
Boni Leonardo,
Guimarães Francisco E. G.,
Pratavieira Sebastião
Publication year - 2020
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.13311
Subject(s) - photobleaching , protoporphyrin ix , photodynamic therapy , photodegradation , photosensitizer , light source , wavelength , phototoxicity , chemistry , photochemistry , optoelectronics , biophysics , optics , protoporphyrin , visible spectrum , fluorescence , materials science , physics , photocatalysis , biology , porphyrin , catalysis , biochemistry , organic chemistry , in vitro
Photodynamic procedures have been used in many applications, ranging from cancer treatment to microorganism inactivation. Photodynamic reactions start with the activation of a photosensitizing molecule with light, leading to the production of cytotoxic molecules that promote cell death. However, establishing the correct light and photosensitizer dosimetry for a broadband light source remains challenging. In this study, we proposed a theoretical mathematical model for the photodegradation of protoporphyrin IX (PpIX), when irradiated by multi‐wavelength light sources. The theoretical model predicts the experimental photobleaching (temporal change in PpIX concentration) of PpIX for different light sources. We showed that photobleaching occurs independently of the light source wavelengths but instead depends only on the number of absorbed photons. The model presented here can be used as an important mathematical approach to better understand current photodynamic therapy protocols and help achieve optimization of the doses delivered.