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Singlet Oxygen Luminescence Image in Blood Vessels During Vascular‐Targeted Photodynamic Therapy
Author(s) -
Lin Lisheng,
Lin Huiyun,
Shen Yi,
Chen Defu,
Gu Ying,
Wilson Brian C.,
Li Buhong
Publication year - 2020
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.13264
Subject(s) - luminescence , singlet oxygen , photodynamic therapy , optical imaging , materials science , chemistry , oxygen , photochemistry , optics , optoelectronics , physics , organic chemistry
Singlet oxygen ( 1 O 2 ) is widely regarded as the main cytotoxic substance that induces the biological damage for photodynamic therapy (PDT). In this study, the previously developed near‐infrared (NIR) optical imaging system was optimized for fast imaging of 1 O 2 luminescence. The optical imaging system enables direct imaging of 1 O 2 luminescence in blood vessels within 2 s during vascular‐targeted PDT (V‐PDT), which makes this system extremely practical for in vivo studies. The dependence of RB concentration on 1 O 2 luminescence image was investigated for V‐PDT, and the data imply that 1270 nm signal is attributed to 1 O 2 luminescence. The imaging system operates with a field of view of 9.60 × 7.68 mm 2 and a spatial resolution of 30 μm, which holds the potential to elucidate the correlation between cumulative 1 O 2 luminescence and vasoconstriction for V‐PDT.