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In vivo Spectroscopic Evaluation of the Intraperitoneal Cavity in Canines
Author(s) -
Cramer Gwendolyn M.,
Sandell Meo Julia,
Finlay Jarod C.,
Zhu Timothy C.,
Busch Theresa M.,
Cengel Keith A.
Publication year - 2020
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.13226
Subject(s) - photodynamic therapy , photobleaching , in vivo , fluorescence , dosimetry , oxygenation , photosensitizer , medicine , biomedical engineering , nuclear medicine , materials science , chemistry , photochemistry , optics , physics , microbiology and biotechnology , organic chemistry , biology
As part of a preclinical trial for the treatment of peritoneal carcinomatosis (PC) with photodynamic therapy (PDT), we have assessed changes in optical properties, tissue oxygenation and drug concentration as a result of benzoporphyrin derivative (BPD)‐mediated PDT using diffuse reflectance and fluorescence measurements. PDT can effectively treat superficial disease spread, but treatment efficacy is influenced by physical properties of the treated tissue which can change over the treatment time. In this study, healthy canines were given BPD and irradiated with 690 nm light during a partial bowel resection, and spectroscopic and fluorescence measurements were made using an in‐house built spectroscopic probe. Hemoglobin concentration, oxygenation and optical properties were determined to be highly heterogeneous between canines and at different anatomical locations within the same subject, so further development of PDT dosimetry systems will need to address this patient and location‐specific dose optimization. Compared to other photosensitizers, we found no apparent BPD photobleaching after PDT.