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Effect of High Intensity Blue Light on Fusobacterium nucleatum Membrane Integrity
Author(s) -
Jeffet Uziel,
Shimon Rachel,
Sterer Nir
Publication year - 2019
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.13151
Subject(s) - fusobacterium nucleatum , bacteria , phototoxicity , chemistry , membrane permeability , microbiology and biotechnology , membrane , fusobacterium , biophysics , biochemistry , biology , porphyromonas gingivalis , bacteroides , genetics , in vitro
Oral malodour is considered to be caused mainly by the production of volatile sulfide compounds ( VSC ) by anaerobic gram‐negative oral bacteria. Previous studies showed that these bacteria were susceptible to blue light phototoxicity mediated by the production of reactive oxygen species ( ROS ). In the present study, we tested the effect of blue light on the integrity Fusobacterium nucleatum 's membrane, cellular proteins and DNA . Bacterial samples were exposed to high intensity blue light for 0, 70, 140 and 280 s ( i.e . fluences of 0, 96, 192 and 384 J cm −2 , respectively). Following light exposure, bacterial samples were examined for membrane damage using fluorescence microscopy, intra‐cellular protein analysis using electrophoresis ( SDS ‐ PAGE ) and DNA fragmentation using ultra–filtration. Results showed that the increasing exposure of bacterial samples to blue light caused increased membrane permeability concomitant with a reduction in intra‐cellular proteins and DNA fragments content. These results suggest that membrane damage is the main effect of high intensity blue light exposure on malodour producing bacteria.