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Effects of HPV Status on Responsiveness to Ionizing Radiation vs Photodynamic Therapy in Head and Neck Cancer Cell lines
Author(s) -
Kessel David,
Cho Won Jin,
Rakowski Joseph,
Kim Harold E.,
Kim HyeongReh C.
Publication year - 2019
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.13150
Subject(s) - photodynamic therapy , apoptosis , cancer research , programmed cell death , cell culture , head and neck squamous cell carcinoma , hela , cancer , phototoxicity , head and neck cancer , ionizing radiation , cell , radiation therapy , medicine , in vitro , biology , chemistry , irradiation , biochemistry , genetics , physics , organic chemistry , nuclear physics
Efficacy of ionizing radiation (I/R) was compared with phototoxic effects of photodynamic therapy (PDT) in vitro using two cell lines derived from patients with head and neck squamous cell carcinoma (HNSCC). A cell line derived from a donor with a human papilloma virus (HPV) infection was more responsive to I/R but significantly less responsive to PDT than a cell line derived from an HPV‐free patient. Cell death after I/R in the HPV(+) cell line was associated with increased DEVDase activity, a hallmark of apoptosis. The HPV(−) line was considerably less responsive to I/R, with DEVDase activity greatly reduced, suggesting an impaired apoptotic program. In contrast, the HPV(−) cells were readily killed by PDT when the ER was among the targets for photodamage. While DEVDase activity was enhanced, the death pathway appears to involve paraptosis until the degree of photodamage reached the LD 99 range. These data suggest that PDT‐induced paraptosis can be a death pathway for cells with an impaired apoptotic program.