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The Growth‐promoting Mechanism of Unusual Spectroscopic Form of LH 2 ( LH 4) from Rhodopseudomonas palustris CGA 009 in Low Light
Author(s) -
Zhao Xiaolan,
Zhao Chungui,
Yang Suping,
Luo Jiafu
Publication year - 2019
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.13134
Subject(s) - rhodopseudomonas palustris , photopigment , mutant , biophysics , fluorescence , biology , chemistry , photochemistry , biochemistry , bacteria , gene , genetics , physics , optics , retinal
The experimental evidence for the growth‐promoting mechanism and the efficiency of energy transfer ( EET ) of LH 4 under low light are still not available. To elucidate the light adaption mechanism of LH 4, we deleted the genes puc BA d involved in the synthesis of the α / β polypeptides of LH 4 in Rhodopseudomonas palustris CGA 009. Compared to wild strain, the growth rate of puc BA d mutant significantly decreased under low light, while there were no significant changes in the growth rate, the contents and compositions of photopigments, absorption spectra of cell lysates under high light. Moreover, the fluorescence quantum efficiency ( FQE ) was used to further compare the EET between LH 2 and LH 4. The FQE in LH 4 increased up to 1.5‐fold than did in LH 2. Collectively, this study showed that LH 4 could provide more and high energetic state photons for promoting bacterial phototrophic growth in response to low‐light environment.