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Comparative Study of the Effects of Ferrochelatase‐si RNA Transfection Mediated by Ultrasound Microbubbles and Polyethyleneimine in Combination with Low‐dose ALA to Enhance Pp IX Accumulation in Human Endometrial Cancer Xenograft Nude Mice Models
Author(s) -
Zhang Xian,
Chen Longfei,
Gao Lvfen,
Gao Xuesong,
Li Nan,
Song Yuwei,
Huang Xinke,
Lin Shaoqiang,
Wang Xiaoyu
Publication year - 2019
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.13076
Subject(s) - microbubbles , transfection , ultrasound , chemistry , fluorescence , ferrochelatase , in vivo , microbiology and biotechnology , fluorescence microscope , rna , medicine , biology , biochemistry , gene , heme , physics , radiology , quantum mechanics , enzyme
Abstract Comparison of the fluorescence intensity caused by the accumulation of Pp IX in endometrial cancer xenografts in nude mice after low‐dose 5‐Aminolevulinic acid (ALA) injection combined with si RNA transfection was mediated by ultrasound microbubbles and polyethyleneimine (PEI) to explore the feasibility of the ultrasound microbubble technique as transfection agents. Knockdown of ferrochelatase (FECH) in human endometrial cancer xenografts in nude mice was performed by transfection with FECH ‐si RNA mediated by PEI and ultrasound microbubbles alone or in combination; then, low‐dose ALA was injected. Subsequently, an in vivo animal imaging system was employed to detect the fluorescence intensity in xenografts. Red fluorescence was observed in xenografts given more than 6.25 mg kg −1 of ALA . When the dose of ALA was greater than 50 mg kg −1 , there was a significant difference in the fluorescence between tumor and other tissues. After the nude mice were transfected with si RNA and treated with low‐dose ALA (1.0 mg kg −1 ), apparent Pp IX fluorescence of the xenografts was observed, and the fluorescence intensity was PEI + ultrasound microbubbles > PEI > ultrasound microbubbles. Ultrasound microbubbles in combination with PEI could generate a higher fluorescence intensity of Pp IX than that obtained with ultrasound microbubbles or PEI alone, and ultrasound microbubbles could wholly or partially replace PEI under certain conditions.