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Spectral Properties of Fluorogenic Thiophene‐derived Triarylmethane Dyes
Author(s) -
Stensrud Kenneth F.,
Zanotti Kimberly J.,
Waggoner Alan S.,
Armitage Bruce A.
Publication year - 2018
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.13037
Subject(s) - thiophene , fluorescence , malachite green , absorption (acoustics) , photochemistry , absorption band , aryl , chemistry , wavelength , excitation wavelength , spectral properties , analytical chemistry (journal) , materials science , optics , computational chemistry , optoelectronics , organic chemistry , physics , alkyl , adsorption
Spectral properties and fluorogenic behaviors of five novel thiophene variants of malachite green (MG), termed MGTs, were determined. Appreciable changes as a function of homologation and substitution pattern, including absorption band positions and intensities and fluorescence quantum yields were observed. In particular, the shorter wavelength y‐band absorption was found to shift over a nearly 200 nm range based on aryl group variation, allowing fine‐tuning of the excitation wavelength for these dyes. In addition, the fluorescence intensity of some MGTs increased significantly (up to 4600‐fold) when the dye was bound to a cognate protein partner, which is potentially useful for cell imaging studies.