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Protective Effect of Baicalin Against TLR4‐mediated UVA‐induced Skin Inflammation
Author(s) -
Sherwani Mohammad Asif,
Yang Kevin,
Jani Aditi,
Abed Reham A.,
Taufique Abdul Karim,
Dosunmu Tolulope Gid,
Yusuf Nabiha
Publication year - 2018
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.13021
Subject(s) - baicalin , tlr4 , inflammation , chemistry , medicine , immunology , chromatography , high performance liquid chromatography
Abstract UVA irradiation is known to cause photoaging via production of reactive oxygen species ( ROS ) and activation of inflammatory processes. Previously, we have demonstrated that baicalin, a plant‐derived flavonoid possessing both antioxidant and anti‐inflammatory activity, protects mouse keratinocytes against damage from UVB irradiation. However, the role of baicalin in vivo has not been well studied, particularly in the setting of UVA irradiation. To explore the protective effects and mechanisms of baicalin treatment in mice after UVA irradiation, mice were exposed to acute and chronic doses of UVA irradiation with or without baicalin or vehicle. Skin samples were collected for histological staining, RNA isolation, flow cytometry and protein extraction. Our results demonstrate the protective effect of baicalin against UVA ‐induced oxidative damage and inflammation in mouse skin. These effects are likely mediated via the TLR 4 pathway, which may serve as a target for photochemoprevention against skin inflammation.