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Effect of Monochromatic Light on Circadian Rhythm of Clock Genes in Chick Pinealocytes
Author(s) -
Ma Shuhui,
Wang Zixu,
Cao Jing,
Dong Yulan,
Chen Yaoxing
Publication year - 2018
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12963
Subject(s) - pinealocyte , circadian rhythm , melatonin , biology , darkness , photoperiodism , pineal gland , medicine , endocrinology , circadian clock , suprachiasmatic nucleus , clock , secretion , microbiology and biotechnology , botany
The avian circadian system is a complex of mutually coupled pacemakers residing in pineal gland, retina and suprachiasmatic nucleus. In this study, the self‐regulation mechanism of pineal circadian rhythm was investigated by culturing chick primary pinealocytes exposed to red light ( RL ), green light ( GL ), blue light ( BL ), white light ( WL ) and constant darkness ( DD ), respectively. All illuminations were set up with a photoperiod of 12 light: 12 dark. The 24‐h expression profiles of seven core clock genes ( cB mal1/2 , cC lock , cC ry1/2 and cP er2/3 ), cA anat and melatonin showed significant circadian oscillation in all groups, except for the loss of cC ry1 rhythm in BL . Compared to WL , GL increased the amplitudes and mesors of positive elements ( cC lock and cB mal1/2 ) and reduced those of negative elements ( cC ry1/2 and cP er2/3 ), in contrast to RL . The temporal patterns of cA anat mRNA and melatonin secretion have always been consistent with the positive genes. Besides, GL advanced the acrophases of the positive elements, cA anat and melatonin, but RL and BL showed the opposite effect. Thereby, GL could promote the secretion of melatonin by enhancing the expressions of positive clock genes and repressing the expressions of negative clock genes.