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Genoprotective Effect of Phyllanthus orbicularis Extract Against UVA, UVB, and Solar Radiation
Author(s) -
Tamayo Marioly Vernhes,
Schuch André Passaglia,
Yagura Teiti,
Gil Luis Baly,
Menck Carlos Frederico Martins,
SánchezLamar Angel
Publication year - 2018
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12939
Subject(s) - dna damage , dna , pyrimidine dimer , chemistry , photolyase , dna glycosylase , agarose gel electrophoresis , biochemistry , dna repair , microbiology and biotechnology , biology
One approach to protect the human skin against harmful effects of solar ultraviolet ( UV ) radiation was to use natural products as photoprotectors. In this work, the extract from specie Phyllanthus orbicularis K was evaluated as a protective agent against the photodamage by UVB , UVA artificial lamps, and environmental sunlight exposure. The plasmid DNA solutions were exposed to radiations using the DNA dosimeter system in the presence of plant extract. The DNA repair enzymes, Escherichia coli Formamidopyrimidine‐ DNA glycosylase (Fpg) and T4 bacteriophage endonuclease V (T4‐endo V), were employed to discriminate oxidized DNA damage and cyclobutane pyrimidine dimers ( CPD ), respectively. The supercoiled and relaxed forms of DNA were separated through electrophoretic migration in agarose gels. These DNA forms were quantified to determine strand break, representing the types of lesion levels. The results showed that, in the presence of P. orbicularis extract, the CPD and oxidative damage were reduced in irradiated DNA samples. The photoprotective effect of extract was more evident for UVB and sunlight radiation than for UVA . This work documented the UV absorbing properties of P. orbicularis aqueous extract and opened up new vistas in its characterization as protective agent against DNA damage induced by environmental sunlight radiation.