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Comparison of Blue and White Lamp Light with Sunlight for Daylight‐Mediated, 5‐ ALA Photodynamic Therapy, in vivo
Author(s) -
Marra Kayla,
LaRochelle Ethan P.,
Chapman M. Shane,
Hoopes P. Jack,
Lukovits Karina,
Maytin Edward V.,
Hasan Tayyaba,
Pogue Brian W.
Publication year - 2018
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12923
Subject(s) - daylight , sunlight , actinic keratosis , photobleaching , photodynamic therapy , artificial light , blue light , light source , chemistry , white light , medicine , optics , fluorescence , physics , illuminance , organic chemistry , basal cell
Daylight‐mediated photodynamic therapy (d‐ PDT ) as a treatment for actinic keratosis ( AK ) is an increasingly common technique due to a significant reduction in pain, leading to better patient tolerability. While past studies have looked at different light sources and delivery methods, this study strives to provide equivalent Pp IX ‐weighted light doses with the hypothesis that artificial light sources could be equally as effective as natural sunlight if their Pp IX ‐weighted fluences were equalized. Normal mouse skin was used as the model to compare blue LED light, metal halide white light and natural sunlight, with minimal incubation time between topical ALA application and the onset of light delivery. A total Pp IX ‐weighted fluence of 20 J eff cm −2 was delivered over 2 h, and the efficacy of response was quantified using three acute bioassays for PDT damage: Pp IX photobleaching, Stat3 crosslinking and quantitative histopathology. These bioassays indicated blue light was slightly inferior to both sunlight and white light, but that the latter two were not significantly different. The results suggest that metal halide white light could be a reasonable alternative to daylight PDT , which should allow a more controlled treatment that is independent of weather and yet should have similar response rates with limited pain during treatment.