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Photobiomodulation in the Metabolism of Lipopolysaccharides‐exposed Epithelial Cells and Gingival Fibroblasts
Author(s) -
Pansani Taisa Nogueira,
Basso Fernanda Gonçalves,
Soares Diana Gabriela,
Turrioni Ana Paula da Silveira,
Hebling Josimeri,
de Souza Costa Carlos Alberto
Publication year - 2018
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12877
Subject(s) - hacat , viability assay , trypan blue , chemokine , wound healing , chemistry , cell culture , fibroblast , cell growth , microbiology and biotechnology , lipopolysaccharide , andrology , cell , immunology , inflammation , biology , in vitro , medicine , biochemistry , genetics
This study assessed the effects of photobiomodulation ( PBM ) to cells previously exposed to lipopolysaccharides ( LPS ). Human gingival fibroblasts ( HGF ) and epithelial cells (HaCaT) were seeded in wells of 24‐well plates containing complete culture medium ( DMEM ). After 24 h, the DMEM was replaced by serum‐free DMEM , and cells were exposed to LPS of Escherichia coli ( E. coli ) (10 μg mL −1 ) for 24, 48, and 72 h. The cells were subjected to specific parameters of phototherapy ( PT ) ( LASERT able—InGaAsP—780 ± 3 nm, 25 mW, 3 J cm −2 ). Cell proliferation (alamarBlue ® ), viability (Trypan Blue) and synthesis of CCL 2 ( ELISA ) were evaluated. Data were statistically analyzed by the Kruskal–Wallis and Mann–Whitney test ( α = 5%). Proliferation and viability of both cell lines decreased after LPS treatment at 48 and 72 h. Enhanced synthesis of CCL 2 by gingival fibroblasts occurred at 24 h, while epithelial cells increased synthesis of this chemokine at 48 and 72 h. PBM enhanced cell proliferation and viability in a time‐dependent manner for both cell lines exposed or not to LPS , while synthesis of CCL 2 by cells exposed to PT decreased over time. PBM caused biomodulatory effects on gingival fibroblasts and epithelial cells previously treated with LPS . These effects may decrease tissue inflammatory response and accelerate wound healing of oral mucosal tissue.