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The Different Resistance of Two Astragalus Plants to UV‐B Stress is Tightly Associated with the Organ‐specific Isoflavone Metabolism
Author(s) -
Liu Yang,
Liu Jia,
Wang Yu,
Abozeid Ann,
Tian DongMei,
Zhang Xiaoning,
Tang ZhongHua
Publication year - 2017
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12841
Subject(s) - metabolism , astragalus , chemistry , biology , biochemistry , medicine , traditional chinese medicine , pathology , alternative medicine
In this work, the changes in isoflavone levels and the expression of genes involved in their biosynthesis were studied in two Astragalus by UPLC ‐ MS and real‐time PCR after 10 days of UV ‐B treatment ( λ max = 313 nm, 804 J m −2 ). Isoflavones were significantly induced by UV ‐B irradiation. The influence might be activated by the regulation of these target genes. Our results indicate that (1) the resistance of Astragalus membranaceus might not be as good as Astragalus mongholicus in the enhanced UV ‐B radiation environment; (2) the enhanced accumulation of calycosin and calycosin‐7‐glucoside with UV ‐B treatment in roots of A. mongholicus might be derived from formononetin which is synthesized in the leaves; (3) the glycosylation process could be stimulated and activated by the enhanced UV ‐B radiation in both A. mongholicus and A. membranaceus . In other words, glycosylation of isoflavones might play a crucial role for two Astragalus plants in response to UV ‐B stress. Overall, this study offered a feasible elicitation strategy to understand the accumulation pattern of isoflavone in A. mongholicus and A. membranaceus , and also provided a reference for the changes in isoflavone levels of Astragalus in UV ‐B enhanced environment in the future.