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Pterocarpus santalinus L. Regulated Ultraviolet B Irradiation‐induced Procollagen Reduction and Matrix Metalloproteinases Expression Through Activation of TGF ‐ β /Smad and Inhibition of the MAPK / AP ‐1 Pathway in Normal Human Dermal Fibroblasts
Author(s) -
Gao Wei,
Lin Pei,
Hwang Eunson,
Wang Yushuai,
Yan Zhengfei,
Ngo Hien T.T.,
Yi TaeHoo
Publication year - 2017
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12835
Subject(s) - mapk/erk pathway , matrix metalloproteinase , smad , reactive oxygen species , chemistry , p38 mitogen activated protein kinases , kinase , photoaging , signal transduction , antioxidant , microbiology and biotechnology , phosphorylation , pharmacology , biochemistry , biology , genetics
Ultraviolet light‐induced reactive oxygen species ( ROS ) damage human skin and prematurely cause aging. A growing body of research is focusing on considering plants and plant‐derived compounds as antiphotoaging therapeutic material. Pterocarpus santalinus L., as an Indian traditional medicine, possesses antidiabetic, anti‐inflammatory and antioxidative effects. Here, we studied the antiphotoaging effects of ethanolic extract of P. santalinus L. heartwood ( EPS ) on ultraviolet radiation B ( UVB )‐irradiated normal human dermal fibroblasts ( NHDF s). Results showed that EPS significantly inhibited the upregulation of matrix metalloproteinases and IL ‐6 caused by UVB irradiation, and suppressed UVB ‐induced phosphorylation of extracellular signal‐regulated kinase, Jun N‐terminal kinase and p38, as well as the activation of AP ‐1 transcription factors. Further study indicated that UVB ‐induced production of MMP ‐1 and IL ‐6 could be inhibited by PD 98059 (an ERK inhibitor) and SP 600125 (A JNK inhibitor), implied that EPS inhibited UVB ‐induced MMP ‐1 and IL ‐6 secretion by inactivating MAPK signaling pathway. In addition, EPS possessed an excellent antioxidant activity, which could increase cytoprotective antioxidants such as HO ‐1, NQ ‐O1 expression by facilitating the nuclear accumulation of Nrf2. Treatment of NHDF s with EPS also recovered UVB ‐induced procollagen type I reduction by activating TGF ‐ β /Smad pathway. These findings demonstrated that EPS had a potential effect against UVB ‐induced skin photoaging.