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NAD (P)H and Flavin Autofluorescence Correlation with ATP in Rat Livers with Different Metabolic Steady‐State Conditions
Author(s) -
Croce Anna C.,
Ferrigno Andrea,
Di Pasqua Laura G.,
Berardo Clarissa,
Bottiroli Giovanni,
Vairetti Mariapia
Publication year - 2017
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12804
Subject(s) - autofluorescence , nad+ kinase , flavin group , chemistry , biochemistry , metabolism , steady state (chemistry) , biophysics , biology , enzyme , fluorescence , physics , quantum mechanics
The monitoring of NAD (P)H and flavin autofluorescence ( AF ) is at the basis of numerous investigations on energy metabolism. Nevertheless, the ability of these AF biomarkers to accurately represent the energy currency, ATP , is poorly explored. Here, we focused on the AF / ATP correlation in lean and fatty livers with different steady‐state metabolic conditions, achieved after organ isolation, preservation and recovery, in a likely dependence on both liver intrinsic metabolic features and externally induced perturbations. Within these eventual, various conditions, a significant correlation was detected between liver NAD (P)H and flavin AF , measured via fiber‐optic probe, and biochemical ATP data, strengthening AF as biomarker of energy metabolism in steady‐state conditions for wide‐ranging experimental and diagnostic applications.