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An In Vitro Model for Fibroblast Photoaging Comparing Single and Repeated UVA Irradiations
Author(s) -
Nakyai Wongnapa,
Saraphanchotiwitthaya Aurasorn,
Viennet Céline,
Humbert Philippe,
Viyoch Jarupa
Publication year - 2017
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12801
Subject(s) - photoaging , fibroblast , fibronectin , procollagen peptidase , chemistry , irradiation , in vitro , skin aging , matrix metalloproteinase , human skin , type i collagen , contraction (grammar) , biophysics , microbiology and biotechnology , andrology , cell , pathology , biochemistry , biology , endocrinology , medicine , dermatology , physics , genetics , nuclear physics
The current method for efficient evaluation of antiphotoaging compounds is an in vitro skin culture model using a single ultraviolet A ( UVA ) irradiation of fibroblasts. However, skin photoaging is caused by repeated exposure to UVA radiation. The objective of this study was to develop an appropriate model for in vitro skin photoaging by comparing the different effects of single (5 J cm −2 ) and repeated exposures (5 J cm −2 × 3 times) of fibroblasts to UVA irradiation. Our results demonstrated that a single and repeated exposure to UVA irradiation had different effects on fibroblasts. In the single UVA ‐irradiated group, collagen lattice contraction and the protein levels of type I procollagen and matrix metalloproteinase‐1 ( MMP ‐1) increased, while the levels of fibronectin and alpha‐smooth muscle actin ( α ‐ SMA ) were unchanged, compared to levels in the non‐ UVA ‐irradiated group (control). In contrast, repeated UVA exposure significantly induced G0/G1 cell cycle arrest, reduced collagen lattice contraction and type I procollagen and fibronectin expression, and increased MMP ‐1 expression. There was no difference in α ‐ SMA expression when comparing repeatedly irradiated and non‐ UVA ‐irradiated fibroblasts. Our findings clearly indicate that repeated UVA irradiation of cells induces malfunctions found in photoaged skin and is an appropriate in vitro skin model of photoaging.