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A Simple Preparation Method for Phytochromobilin
Author(s) -
Lu Lu,
Zhao BaoQing,
Miao Dan,
Ding WenLong,
Zhou Ming,
Scheer Hugo,
Zhao KaiHong
Publication year - 2017
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12710
Subject(s) - phycocyanobilin , phytochrome , chromophore , phycobiliprotein , chemistry , linker , biochemistry , escherichia coli , cyanobacteria , biophysics , phycocyanin , biology , bacteria , botany , photochemistry , gene , red light , genetics , computer science , operating system
Phytochromobilin (PΦB), the chromophore of plant phytochromes, is difficult to isolate because phytochromes occur at very low concentrations in plants. It is, therefore, frequently replaced in plant phytochrome studies by phycocyanobilin, which is abundant in cyanobacteria. PΦB is also an attractive chromophore for far‐red emitting chromoproteins. In this work, we design and optimize a simple method to efficiently isolate useful quantities of PΦB: The chromophore is generated in Escherichia coli and transiently bound to a tailored chromophore‐binding domain of ApcE2, the apo‐protein of a core‐membrane linker, from which it can subsequently be released. The ease and effectiveness of this method hinges not only on the enhanced biosynthesis of PΦB in the presence of the ApcE2 construct from Synechococcus sp. PCC 7335, but also on the noncovalent binding of the pigment to its apo‐protein. The isolated PΦB was successfully incorporated into phytochrome‐related assemblies, and furthermore, the noncovalently bound PΦB could be transferred directly from the ApcE2 construct to the apo‐proteins of phytochromes, cyanobacteriochromes and phycobiliproteins, without loss of relevant biological activity.