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In‐Planta Expression: Searching for the Genuine Chromophores of Cryptochrome‐3 from Arabidopsis thaliana
Author(s) -
Gärtner Wolfgang
Publication year - 2017
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12693
Subject(s) - chromophore , cryptochrome , flavin group , cofactor , arabidopsis thaliana , arabidopsis , chemistry , heterologous expression , biochemistry , photochemistry , enzyme , recombinant dna , gene , circadian clock , mutant
Göbel et al . present in this issue an exemplary study of identification of chromophores from Arabidopsis thaliana cryptochrome‐3. Usually taken for granted, proteins and cofactors, respective chromophores, from heterologous expression are considered identical to material isolated from their genuine host. Cryptochromes carry two chromophores, an antenna cofactor and a functional flavin chromophore, both noncovalently embedded into the protein. In particular the antenna chromophore is loosely bound and often lost during protein purification. The authors identify from plant‐extracted Cry3 unambiguously N 5 ,N 10 ‐methenyltetrahydrofolate as antenna chromophore and flavin adenine dinucleotide as the functional chromophore.