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Bimodal Targeting Using Sulfonated, Mannosylated PEI for Combined Gene Delivery and Photodynamic Therapy
Author(s) -
Chitgupi Upendra,
Li Yi,
Chen Mingfu,
Shao Shuai,
Beitelshees Marie,
Tan Myles Joshua,
Neelamegham Sriram,
Pfeifer Blaine A.,
Jones Charles,
Lovell Jonathan F.
Publication year - 2017
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12688
Subject(s) - photodynamic therapy , photosensitizer , transfection , cytotoxicity , gene delivery , genetic enhancement , chemistry , cancer cell , cationic polymerization , cancer research , microbiology and biotechnology , gene , cancer , biochemistry , biology , in vitro , organic chemistry , genetics
Photodynamic therapy ( PDT ) and gene delivery have both been used to target both cancer cells and tumor‐associated macrophages ( TAM s). Given the complex nature of tumor tissue, there could be merit in combining these strategies simultaneously. In this study, we developed a bimodal targeting approach to both cancer cells and macrophages, employing materials conducive to both gene delivery and PDT . Polymers libraries were created that consisted of cationic polyethyleneimine ( PEI ) conjugated to the photosensitizer pyropheophorbide‐a, with sulfonation (to target selectin‐expressing cells) and mannosylation (to target TAM s). Polyplexes, consisting of these polymers electrostatically bound to DNA , were analyzed for transfection efficacy and cytotoxicity toward epithelial cells and macrophages to assess dual‐targeting. This study provides preliminary proof of principle for using modified PEI for targeted gene delivery and PDT .