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Hybrid Minimal Core Streptavidin–Obelin as a Versatile Reporter for Bioluminescence‐based Bioassay
Author(s) -
Bashmakova Eugenia E.,
Krasitskaya Vasilisa V.,
Kudryavtsev Alexander N.,
Grigorenko Vitaly G.,
Frank Ludmila A.
Publication year - 2016
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12648
Subject(s) - streptavidin , bioluminescence , photoprotein , conjugate , chemistry , biophysics , chromatography , combinatorial chemistry , biochemistry , biology , biotin , mathematical analysis , mathematics
Ca 2+ ‐regulated photoprotein obelin was genetically fused with a minimum‐sized core streptavidin. Hybrid protein ( SAV – OL ) was produced by bacterial expression and applied as a specific bioluminescent probe in diverse solid‐phase assays. The obtained results clearly demonstrate specific activity of each domain indicating its proper folding with favorable space orientation. SAV – OL has been shown to be a much more sensitive label than the chemical conjugate of a full‐length streptavidin with obelin.