Hybrid Minimal Core Streptavidin–Obelin as a Versatile Reporter for Bioluminescence‐based Bioassay | Zendy

Bashmakova Eugenia E. | Zendy; Krasitskaya Vasilisa V. | Zendy; Kudryavtsev Alexander N. | Zendy; Grigorenko Vitaly G. | Zendy; Frank Ludmila A. | Zendy

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Hybrid Minimal Core Streptavidin–Obelin as a Versatile Reporter for Bioluminescence‐based Bioassay

Author(s) -

Bashmakova Eugenia E.,

Krasitskaya Vasilisa V.,

Kudryavtsev Alexander N.,

Grigorenko Vitaly G.,

Frank Ludmila A.

Publication year - 2016

Publication title -

photochemistry and photobiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.818

H-Index - 131

eISSN - 1751-1097

pISSN - 0031-8655

DOI - 10.1111/php.12648

Subject(s) - streptavidin , bioluminescence , photoprotein , conjugate , chemistry , biophysics , chromatography , combinatorial chemistry , biochemistry , biology , biotin , mathematical analysis , mathematics

Ca 2+ ‐regulated photoprotein obelin was genetically fused with a minimum‐sized core streptavidin. Hybrid protein ( SAV – OL ) was produced by bacterial expression and applied as a specific bioluminescent probe in diverse solid‐phase assays. The obtained results clearly demonstrate specific activity of each domain indicating its proper folding with favorable space orientation. SAV – OL has been shown to be a much more sensitive label than the chemical conjugate of a full‐length streptavidin with obelin.

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