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Near–Infrared‐Responsive Peptide that Targets Collagen Fibrils to Induce Cytotoxicity
Author(s) -
Honda Masayuki,
Odawara Aoi,
Suzuki Ikuro,
Shimada Morio,
Yoshikawa Kohki,
Okada Tomoko
Publication year - 2016
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12621
Subject(s) - cytotoxicity , phototoxicity , chemistry , peptide , fibril , biophysics , conjugate , microbiology and biotechnology , biochemistry , in vitro , biology , mathematical analysis , mathematics
A novel conjugate, PHG 10 dye, was synthesized using a collagen peptide and a near–infrared ( NIR )‐responsive dye to achieve targeted cytotoxicity. The collagen peptide motif, ‐(Pro‐Hyp‐Gly) 10 ‐ ( PHG 10), was incorporated for targeting collagen fibrils that are excessively produced by activated fibroblasts around tumor cells. PHG 10 dye was purified by HPLC and identified by MALDI ‐ MS . The phototoxicity and cytotoxicity of PHG 10 dye were examined using human glioma cells ( HGC s). Fluorescent images indicated that PHG 10 dye preferably assembled to collagen‐coated HGC s compared with noncoated HGC s. Under irradiation with NIR light, effective cytotoxicity was observed on collagen‐coated HGC s within 20 min. Because phototoxicity and cytotoxicity are dependent on the assembled amount of PHG 10 dye, the targeting of collagen fibrils by the collagen peptide motif PHG 10 is assured.