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Differential Laser‐Induced Perturbation Spectroscopy for Analysis of Mixtures of the Fluorophores l ‐Phenylalanine, l ‐Tyrosine and l ‐Tryptophan Using a Fluorescence Probe
Author(s) -
Oztekin Erman K.,
Hahn David W.
Publication year - 2016
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12618
Subject(s) - phenylalanine , tryptophan , tyrosine , chemistry , fluorescence , fluorescence spectroscopy , physics , amino acid , biochemistry , optics
Abstract Quantitative detection of common endogenous fluorophores is accomplished using differential laser‐induced perturbation spectroscopy ( DLIPS ) with a 193‐nm UV fluorescence probe and various UV perturbation wavelengths. In this study, DLIPS is explored as an alternative to traditional fluorescence spectroscopy alone, with a goal of exploring natural fluorophores pursuant to biological samples and tissue analysis. To this end, aromatic amino acids, namely, l ‐phenylalanine, l ‐tyrosine and l ‐tryptophan are mixed with differing mass ratios and then classified with various DLIPS schemes. Classification with a traditional fluorescence probe is used as a benchmark. The results show a 20% improvement in classification performance of the DLIPS method over the traditional fluorescence method using partial least squares ( PLS ) analysis. Additional multivariate analyses are explored, and the relevant photochemistry is elucidated in the context of perturbation wavelengths. We conclude that DLIPS is a promising biosensing approach with potential for in vivo analysis given the current findings with fluorophores relevant to biological tissues.