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Reduced Levels of Tissue Inhibitors of Metalloproteinases in UVB ‐Irradiated Corneal Epithelium
Author(s) -
Ardan Taras,
Němcová Lucie,
Bohuslavová Božena,
Klezlová Adéla,
Popelka Štěpán,
Studenovská Hana,
Hrnčiarová Eva,
Čejková Jitka,
Motlík Jan
Publication year - 2016
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12612
Subject(s) - matrix metalloproteinase , corneal epithelium , epithelium , cornea , extracellular matrix , proteolysis , immunohistochemistry , proteolytic enzymes , chemistry , endogeny , matrix (chemical analysis) , biology , pathology , microbiology and biotechnology , enzyme , immunology , biochemistry , medicine , neuroscience , chromatography
Tissue inhibitors of metalloproteinases ( TIMP s) are the major endogenous regulators of metalloproteinase activity in tissues. TIMP s are able to inhibit activity of all known matrix metalloproteinases ( MMP s) and thus participate in controlling extracellular matrix synthesis and degradation. We showed previously elevated expressions of MMP s in the rabbit corneal epithelium upon UVB exposure and suggested that these enzymes might be involved in corneal destruction caused by excessive proteolysis. The aim of this study was to investigate TIMP s in the corneal epithelium after UV irradiation using immunohistochemical and biochemical methods. We found that as compared to control rabbit corneas where relatively high levels of TIMP s were present in the epithelium, repeated irradiation of the cornea with UVB rays (not with UVA rays of similar doses) significantly decreased TIMP s in corneal epithelial cells. The results of this study point to the suggestion that the decrease in TIMP s in the corneal epithelium after UVB irradiation contributes to increased proteolytic activity of MMP s in UVB ‐irradiated corneal epithelium found previously.