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Identification of a Fluorescent Protein from Rhacostoma Atlantica
Author(s) -
Tota Michael R.,
Allen Jeanna M.,
Karolin Jan O.,
Geddes Chris D.,
Ward William W.
Publication year - 2016
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12609
Subject(s) - fluorescence , chromophore , quantum yield , glycine , amino acid , tyrosine , absorption (acoustics) , alanine , chemistry , molar absorptivity , green fluorescent protein , biology , biochemistry , photochemistry , optics , gene , physics
We have cloned a novel fluorescent protein from the jellyfish Rhacostoma atlantica . The closest known related fluorescent protein is the Phialidium yellow fluorescent protein, with only a 55% amino acid sequence identity. A somewhat unusual alanine–tyrosine–glycine amino acid sequence forms the presumed chromophore of the novel protein. The protein has an absorption peak at 466 nm and a fluorescence emission peak at 498 nm. The fluorescence quantum yield was measured to be 0.77 and the extinction coefficient is 58 200 M −1 cm −1 . Several mutations were identified that shift the absorption peak to about 494 nm and the emission peak to between 512 and 514 nm.