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Short‐term UV ‐ B Dose Stimulates Production of Protective Metabolites in M atricaria chamomilla Leaves
Author(s) -
Petruľová Veronika,
Dučaiová Zuzana,
Repčák Miroslav
Publication year - 2014
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12300
Subject(s) - chemistry , phenylalanine ammonia lyase , phenols , phenylalanine , biosynthesis , chlorogenic acid , biochemistry , enzyme , irradiation , food science , peroxidase , amino acid , physics , nuclear physics
Physiological response of two cultivars of M atricaria chamomilla plants on UV irradiation was studied. The impact of used short‐time UV dose was evaluated in three time points; 2, 24 and 48 h after irradiation. Used UV irradiation immediately resulted in changes in plant oxidative status monitored as increased concentration of H 2 O 2 . Decrease in chlorophyll a and b indicated the impact on photosynthetic apparatus. For phenolic secondary metabolites, an increase in total soluble phenols and A l C l 3 ‐reactive flavonols was observed. The activity of main phenolic enzyme, phenylalanine ammonia‐lyase, increased with time after irradiation. Significant changes, mainly decreasing trends, in the content of free coumarins and their glycosidic precursors were observed. Enhanced accumulation in chlorogenic and 1,5‐dicaffeoylquinic acid and in ( Z )‐isoform of dicycloethers was detected. From these results, the redirecting precursors of coumarin biosynthesis to biosynthesis of substances with higher antioxidative potential can be assumed. Different reactions in diploid and tetraploid plants were recorded, too.