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Comparative Antibacterial Efficacy of Photodynamic Therapy and Ultrasonic Irrigation Against E nterococcus faecalis In Vitro
Author(s) -
Wang Yanhuang,
Huang Xiaojing
Publication year - 2014
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12293
Subject(s) - enterococcus faecalis , root canal , photodynamic therapy , ultrasonic sensor , bacteria , colony forming unit , microbiology and biotechnology , chemistry , irrigation , dentistry , medicine , biology , staphylococcus aureus , agronomy , radiology , genetics , organic chemistry
Enterococcus faecali s poses a challenge to the efficacy of traditional root canal disinfection methods. This study was aimed to establish a synergistic root canal disinfection strategy combining ultrasonic irrigation with photodynamic therapy ( PDT ) together and to test its antibacterial efficacy against E . faecalis . Twenty‐seven bovine root canals infected with E . faecalis were randomly divided into three groups and treated with different disinfection methods as follows: ultrasonic irrigation with 2.5% Na OC l, methylene blue ( MB )‐mediated PDT , or combined ultrasonic irrigation and PDT as described above. Quantification of E . faecalis was performed on the root canals before and immediately after the disinfection treatment. Residual bacteria were determined by counting colony‐forming units. Samples were randomly selected from the three groups, and the morphology of residual bacteria inside the dentinal tubules was studied by scanning electron microscopy. The number of surviving E . faecalis in the group treated with the combination method was significantly lower ( P < 0.05) than those in the ultrasonic irrigation‐treated or PDT ‐treated groups. Similar results were found in the morphological studies of the three groups. The results of our study highlighted the importance of combination of ultrasonic irrigation and PDT to produce significant antibacterial efficacy against E . faecalis during root canal disinfection.