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Effects of white light‐emitting diode ( LED ) light exposure with different Correlated Color Temperatures ( CCT s) on human lens epithelial cells in culture
Author(s) -
Xie Chen,
Li Xiuyi,
Tong Jianping,
Gu Yangshun,
Shen Ye
Publication year - 2014
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12250
Subject(s) - white light , lens (geology) , optoelectronics , chemistry , biophysics , microbiology and biotechnology , optics , materials science , biology , physics
Cataract is the major cause for legal blindness in the world. Oxidative stress on the lens epithelial cells ( hLEC s) is the most important factor in cataract formation. Cumulative light‐exposure from widely used light‐emitting diodes (LEDs) may pose a potential oxidative threat to the lens epithelium, due to the high‐energy blue light component in the white‐light emission from diodes. In the interest of perfecting biosafety standards for LED domestic lighting, this study analyzed the photobiological effect of white LED light with different correlated color temperatures (CCTs) on cultured hLEC s. The hLEC s were cultured and cumulatively exposed to multichromatic white LED light with CCTs of 2954, 5624, and 7378 K. Cell viability of hLEC s was measured by Cell Counting Kit‐8 (CCK‐8) assay. DNA damage was determined by alkaline comet assay. Intracellular reactive oxygen species (ROS) generation, cell cycle, and apoptosis were quantified by flow cytometry. Compared with 2954 and 5624 K LED light, LED light having a CCT of 7378 K caused overproduction of intracellular ROS and severe DNA damage, which triggered G 2 /M arrest and apoptosis. These results indicate that white LEDs with a high CCT could cause significant photobiological damage to hLEC s.