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Photosensitive and Biomimetic Core–Shell Nanofibrous Scaffolds as Wound Dressing
Author(s) -
Jin Guorui,
Prabhakaran Molamma P.,
Ramakrishna Seeram
Publication year - 2014
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12238
Subject(s) - nanofiber , gelatin , wound healing , extracellular matrix , biomedical engineering , hyaluronic acid , materials science , scaffold , chronic wound , chemistry , biophysics , nanotechnology , surgery , anatomy , medicine , biochemistry , biology
Tissue engineered skin grafts that mimic the native extracellular matrix of skin has gained huge popularity among clinicians since they increase the survival rate of the patients. Phototherapy shows promising results with respect to acute and chronic pain relief, treatment of inflammatory conditions and promotion of wound healing. Here, we encapsulated a photosensitive polymer poly (3‐hexylthiophene) (P3 HT ) and epidermal growth factor in the core–shell‐structured Gelatin/poly(L‐lactic acid)‐co‐poly‐( ε ‐caprolactone) nanofibers [Gel/ PLLCL /P3 GF (cs)] by coaxial spinning and studied the potential application of the Gel/ PLLCL /P3 GF (cs) nanofibrous scaffold as a novel skin graft. The proliferation of fibroblasts was significantly improved on Gel/ PLLCL /P3 GF (cs) under light stimulation compared to fibroblasts on the same scaffold under dark condition. Studies on the in vitro wound healing ability of Gel/ PLLCL /P3 GF (cs) showed complete closure of wound after 9 days under “light stimulation” too. Furthermore, the potential of adipose‐derived stem cells ( ASC s) to differentiate to epidermal cells on Gel/ PLLCL /P3 GF (cs) was evaluated. The differentiated ASC s with keratinocytes morphology were only found on the light stimulated Gel/ PLLCL /P3 GF (cs). Our results suggest that the photosensitive core–shell Gel/ PLLCL /P3 GF (cs) nanofibers could be a novel substrate to aid in the reestablishment of skin architecture.