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Scrambled Self‐Assembly of Bacteriochlorophylls c and e in Aqueous Triton X‐100 Micelles
Author(s) -
Saga Yoshitaka,
Saiki Tatsuya,
Takahashi Naoya,
Shibata Yutaka,
Tamiaki Hitoshi
Publication year - 2013
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12219
Subject(s) - bacteriochlorophyll , micelle , chemistry , aqueous solution , pigment , absorption (acoustics) , triton x 100 , absorption spectroscopy , spectral line , j aggregate , photochemistry , materials science , organic chemistry , pulmonary surfactant , molecule , physics , optics , biochemistry , astronomy , composite material
Bacteriochlorophyll ( BC hl) e was coassembled with BC hl c in Triton X‐100 micelles in aqueous solutions. The Q y absorption bands of the coaggregates were positioned between those of aggregates consisting solely of BC hl c or e . The electronic absorption spectra of the coaggregates could not be reproduced by linear combinations of the spectra of the aggregates consisting solely of each pigment, but they were in line with the simulated spectra for the self‐aggregates in which both BC hls were randomly distributed. These suggest that BC hls c and e are not spatially separated; they are homogenously distributed over the self‐aggregates to give electronic spectra that are different from those of the aggregate consisting solely of each pigment. Deaggregation of the scrambled self‐aggregates by excess Triton X‐100 did not produce any spectral components assigned to an aggregate consisting solely of either BC hl c or e . Acid‐induced decomposition of the scrambled aggregates showed different kinetics from those of the aggregates consisting solely of each pigment. These also support the homogeneous distribution of BC hls c and e in the scrambled self‐aggregates. These results will be useful to investigate the major light‐harvesting antenna systems of green photosynthetic bacteria that contain two kinds of chlorosomal BC hls.