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Antimicrobial Photodynamic Efficiency of Novel Cationic Porphyrins towards Periodontal Gram‐positive and Gram‐negative Pathogenic Bacteria
Author(s) -
Prasanth Chandra Sekhar,
Karunakaran Suneesh C.,
Paul Albish K.,
Kussovski Vesselin,
Mantareva Vanya,
Ramaiah Danaboyina,
Selvaraj Leslie,
Angelov Ivan,
Avramov Latchezar,
Nandakumar Krishnankutty,
Subhash Narayanan
Publication year - 2013
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12198
Subject(s) - fusobacterium nucleatum , antimicrobial , singlet oxygen , photodynamic therapy , chemistry , microbiology and biotechnology , porphyrin , gram negative bacteria , bacteria , cationic polymerization , photochemistry , porphyromonas gingivalis , biology , biochemistry , organic chemistry , escherichia coli , oxygen , genetics , gene
The Gram‐negative Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum are major causative agents of aggressive periodontal disease. Due to increase in the number of antibiotic‐resistant bacteria, antimicrobial Photodynamic therapy ( aPDT ) seems to be a plausible alternative. In this work, photosensitization was performed on Gram‐positive and Gram‐negative bacteria in pure culture using new‐age cationic porphyrins, namely mesoimidazolium‐substituted porphyrin derivative ( ImP ) and pyridinium‐substituted porphyrin derivative ( PyP ). The photophysical properties of both the sensitizers including absorption, fluorescence emission, quantum yields of the triplet excited states and singlet oxygen generation efficiencies were evaluated in the context of aPDT application. The studied porphyrins exhibited high ability to accumulate into bacterial cells with complete penetration into early stage biofilms. As compared with ImP, PyP was found to be more effective for photoinactivation of bacterial strains associated with periodontitis, without any signs of dark toxicity, owing to its high photocytotoxicity.