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Effect of Laser Phototherapy on Enzymatic Activity of Salivary Glands of Hamsters Treated with 5‐Fluorouracil
Author(s) -
Campos Luana,
Nicolau José,
AranaChavez Victor E.,
Simões Alyne
Publication year - 2013
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12195
Subject(s) - catalase , superoxide dismutase , fluorouracil , lactate dehydrogenase , chemistry , antioxidant , oxidative stress , enzyme , endocrinology , medicine , peroxidase , saliva , chemotherapy , pharmacology , biochemistry
The chemotherapeutic agent 5‐Fluorouracil (5‐ FU ) can induce salivary gland hypofunction ( SGH ); however, previous studies did not reach final conclusions on the influence of this drug on glandular tissue. Thus, the aim of this study was to investigate the effect of 5‐ FU on submandibular ( SM s) and sublingual glands ( SL s), as well as, the effect of laser phototherapy ( LPT ) on SGH induced by 5‐ FU . Eighty‐five hamsters were divided into three groups: control (C), chemotherapy ( CT ) and laser (L), and the SGH was induced by two injections of 5‐ FU in groups CT and L. The irradiation was performed using a diode ( λ 780 nm/20 mW/5 J cm −2 /0.2 J and 10 s per point/spot size of 0.04 cm 2 ) and applied daily. On the euthanasia day, SM s and SL s were removed and biochemical analyses were carried out. The lactate dehydrogenase activity was increased in group CT when compared with group C for SL s and SM s ( P < 0.05). In addition, the peroxidase and catalase activities were increased and superoxide dismutase was decreased by 5‐ FU ( P < 0.05). However, LPT appears to be a protective mechanism against oxidative stress, tending to alter the activity of these antioxidant enzymes, suggesting LPT as a promising therapy to modulate the 5‐ FU harmful effect.