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Interaction of Retinoic Acid Radical Cation with Lysozyme and Antioxidants: Laser Flash Photolysis Study in Microemulsion
Author(s) -
Li Kun,
Wang Mei,
Wang Ting,
Sun Dongmei,
Zhu Rongrong,
Sun Xiaoyu,
Wu Xianzheng,
Wang ShiLong
Publication year - 2013
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12128
Subject(s) - chemistry , flash photolysis , microemulsion , curcumin , lysozyme , radical , photochemistry , retinoic acid , nitrosation , antioxidant , tryptophan , quenching (fluorescence) , biochemistry , reaction rate constant , kinetics , fluorescence , pulmonary surfactant , physics , amino acid , quantum mechanics , gene
All‐trans retinoic acid ( ATRA ) plays essential roles in the normal biological processes and the treatment of cancer and skin diseases. Considering its photosensitive property, many studies have been focused on the photochemistry of ATRA . In this study, we investigated the transient phenomena in the laser flash photolysis ( LFP ) of ATRA in microemulsion to further understand the photochemistry of ATRA . Results show that 355 nm LFP of ATRA in both acidic and alkaline conditions leads to the generation of retinoic acid cation radicals ( ATRA •+ ) via biphotonic processes. The employment of microemulsion system allows us to investigate the reaction of hydrophobic ATRA •+ with molecules of different polarity. Therefore, we studied the reaction activity of ATRA •+ to many hydrophobic and hydrophilic molecules. Results show that ATRA •+ can efficiently interact with lysozyme, tyrosine, tryptophan and many antioxidants, such as curcumin (Cur), vitamin C ( VC ) and gallic acid ( GA ). The apparent rate constants of these reactions were measured and compared. These findings suggest that ATRA •+ is a reactive transient product which may pose damage to lysozyme, and antioxidants, such as Cur, VC and GA , may inactivate ATRA •+ by efficient quenching reactions.