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Morphological and Structural Changes in Lung Tissue Infected by Paracoccidioides brasiliensis : FTIR Photoacoustic Spectroscopy and Histological Analysis
Author(s) -
Morato Edilaine M.,
Morais Gutierrez R.,
Sato Francielle,
Medina Antonio N.,
Svidzinski Terezinha I. E.,
Baesso Mauro L.,
Hernandes Luzmarina
Publication year - 2013
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12110
Subject(s) - paracoccidioides brasiliensis , fourier transform infrared spectroscopy , photoacoustic spectroscopy , photoacoustic imaging in biomedicine , paracoccidioidomycosis , chemistry , microbiology and biotechnology , spectroscopy , biology , optics , physics , quantum mechanics
Abstract This study evaluated physical, chemical and morphological changes in lungs of mice infected with Paracoccidioides brasiliensis . The animals were inoculated with 0.1 mL of fungal suspension of the P. brasiliensis 18 isolate and were euthanized 1, 2, 4 and 8 weeks after inoculation. The upper left lobe of the lung was isolated, fixed and processed for paraffin embedding. The sections were stained with H&E for histopathological study, with Gomori‐Grocott to locate and identify the fungus, and with TUNEL immunostaining to detect the occurrence of programmed cell death. The lower and middle right lobes were analyzed by Fourier Transform Infrared Photoacoustic Spectrocopy (FTIR‐PAS) to investigate physical and chemical features of the infected lungs. The results showed that lungs infected by P. brasiliensis underwent structural changes that varied according to the time period analyzed, and that changes in the absorption bands of different chemical groups resulted from these morphological changes. The results suggest that the combination of FTIR‐PAS spectroscopy with morphological evaluation is an effective procedure for the study of paracoccidioidomycosis, one of the most important systemic mycoses that can damage the lung architecture and consequently impair the respiratory function.