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Protective Effect of Tropical Highland Blackberry Juice ( Rubus adenotrichos Schltdl.) Against UVB ‐Mediated Damage in Human Epidermal Keratinocytes and in a Reconstituted Skin Equivalent Model
Author(s) -
CalvoCastro Laura,
Syed Deeba N.,
Chamcheu Jean C.,
Vilela Fernanda M. P.,
Pérez Ana M.,
Vaillant Fabrice,
Rojas Miguel,
Mukhtar Hasan
Publication year - 2013
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12104
Subject(s) - pyrimidine dimer , chemistry , human skin , photoprotection , antioxidant , dna damage , poly adp ribose polymerase , apoptosis , in vivo , reactive oxygen species , biochemistry , microbiology and biotechnology , biology , enzyme , dna , polymerase , genetics , photosynthesis
Solar ultraviolet ( UV ) radiation, particularly its UVB (280–320 nm) spectrum, is the primary environmental stimulus leading to skin carcinogenesis. Several botanical species with antioxidant properties have shown photochemopreventive effects against UVB damage. Costa Rica's tropical highland blackberry ( R ubus adenotrichos ) contains important levels of phenolic compounds, mainly ellagitannins and anthocyanins, with strong antioxidant properties. In this study, we examined the photochemopreventive effect of R . adenotrichos blackberry juice ( BBJ ) on UVB ‐mediated responses in human epidermal keratinocytes and in a three‐dimensional (3D) reconstituted normal human skin equivalent ( SE ). Pretreatment (2 h) and posttreatment (24 h) of normal human epidermal keratinocytes ( NHEK s) with BBJ reduced UVB (25 mJ cm −2 )‐mediated (1) cyclobutane pyrimidine dimers (CPDs) and (2) 8‐oxo‐7,8‐dihydro‐2′‐deoxyguanosine (8‐oxodG) formation. Furthermore, treatment of NHEK s with BBJ increased UVB ‐mediated (1) poly(ADP‐ribose) polymerase cleavage and (2) activation of caspases 3, 8 and 9. Thus, BBJ seems to alleviate UVB ‐induced effects by reducing DNA damage and increasing apoptosis of damaged cells. To establish the in vivo significance of these findings to human skin, immunohistochemistry studies were performed in a 3D SE model, where BBJ was also found to decrease CPD s formation. These data suggest that BBJ may be developed as an agent to ameliorate UV ‐induced skin damage.