Premium
Photoinactivation of Bacteria Attached to Glass and Acrylic Surfaces by 405 nm Light: Potential Application for Biofilm Decontamination
Author(s) -
McKenzie Karen,
Maclean Michelle,
Timoshkin Igor V.,
Endarko Endarko,
MacGregor Scott J.,
Anderson John G.
Publication year - 2013
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12077
Subject(s) - biofilm , microbiology and biotechnology , bacteria , listeria monocytogenes , human decontamination , escherichia coli , pseudomonas aeruginosa , chemistry , staphylococcus aureus , listeria , microorganism , biology , medicine , biochemistry , genetics , pathology , gene
Attachment of bacteria to surfaces and subsequent biofilm formation remains a major cause of cross‐contamination capable of inducing both food‐related illness and nosocomial infections. Resistance to many current disinfection technologies means facilitating their removal is often difficult. The aim of this study was to investigate the efficacy of 405 nm light for inactivation of bacterial attached as biofilms to glass and acrylic. Escherichia coli biofilms (10 3 –10 8 CFU mL −1 ) were generated on glass and acrylic surfaces and exposed for increasing times to 405 nm light (5–60 min) at ca 140 mW cm −2 . Successful inactivation of biofilms has been demonstrated, with results highlighting complete/near‐complete inactivation (up to 5 log 10 reduction on acrylic and 7 log 10 on glass). Results also highlight that inactivation of bacterial biofilms could be achieved whether the biofilm was on the upper “directly exposed” surface or “indirectly exposed” underside surface. Statistically significant inactivation was also shown with a range of other microorganisms associated with biofilm formation ( Staphylococcus aureus, Pseudomonas aeruginosa and Listeria monocytogenes ). Results from this study have demonstrated significant inactivation of bacteria ranging from monolayers to densely populated biofilms using 405 nm light, highlighting that with further development this technology may have potential applications for biofilm decontamination in food and clinical settings.