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Reversible Conformational Switching of i‐Motif DNA Studied by Fluorescence Spectroscopy
Author(s) -
Choi Jungkweon,
Majima Tetsuro
Publication year - 2013
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/php.12042
Subject(s) - förster resonance energy transfer , dna , fluorescence , biophysics , chemistry , quenching (fluorescence) , cytosine , structural motif , biology , biochemistry , physics , quantum mechanics
Non‐B DNA s, which can form unique structures other than double helix of B‐ DNA , have attracted considerable attention from scientists in various fields including biology, chemistry and physics etc. Among them, i‐motif DNA , which is formed from cytosine (C)‐rich sequences found in telomeric DNA and the promoter region of oncogenes, has been extensively investigated as a signpost and controller for the oncogene expression at the transcription level and as a promising material in nanotechnology. Fluorescence techniques such as fluorescence resonance energy transfer ( FRET ) and the fluorescence quenching are important for studying DNA and in particular for the visualization of reversible conformational switching of i‐motif DNA that is triggered by the protonation. Here, we review the latest studies on the conformational dynamics of i‐motif DNA as well as the application of FRET and fluorescence quenching techniques to the visualization of reversible conformational switching of i‐motif DNA in nano‐biotechnology.