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Starvation‐induced autophagy in Spodoptera frugiperda Sf9 ovarian cells
Author(s) -
Yang Ruopeng,
Shang Xiyu,
Wang Chuanming,
Li Mijie,
Jiang Chengyan,
Lu Haiju,
Kong Qiong,
Gao Bo,
Yang Wanying,
Cao Yang,
Xie Kun
Publication year - 2019
Publication title -
physiological entomology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 57
eISSN - 1365-3032
pISSN - 0307-6962
DOI - 10.1111/phen.12279
Subject(s) - spodoptera , biology , autophagy , starvation , sf9 , botany , zoology , endocrinology , genetics , gene , recombinant dna , apoptosis
Autophagy controls insect development and can be targeted for pest control in agriculture. In the present study, starvation‐induced autophagy is investigated in the insect species Spodoptera frugiperda . Bioinformatics analysis and a search of the EST database (http://bioweb.ensam.inra.fr/spodobase) identifies a putative ATG8 gene of S. frugiperda . To generate a biomarker of autophagosome, the DNA sequence encoding the open reading frame of this gene is amplified and cloned into a pIEX‐4‐ mCherry‐EGFP‐SfATG8 recombinant vector. Sf9 cells are then transfected with this expression vector and starved in phosphate‐buffered saline solution for 4 h to induce autophagy, which is examined by LysoTracker staining (Life Technologies, Grand Island, New York), western blotting and fluorescence microscopy. The results obtained show that starvation stimulates lipidation of SfATG8‐PE and the formation of autophagosomes, providing a foundation for further research with respect to autophagy in insects.